Urine Collection from a Catheter for Home UTI Testing

Why This Matters 

Collecting a clean, fresh urine sample is essential for accurate UTI testing. Poor technique can lead to false results or the need to repeat the test.  Surveillance testing is not advisable if patient is not symptomatic. 

Signs of infection for Catheterized Patients 

• Altered Mental Status 

• Hematuria 

• Dysuria 

• Bladder spasms 

• Flank Pain 

• Nausea 

• Fever  

What You’ll Need 

• Sterile specimen container (provided in your test kit) 

•  Alcohol or antiseptic wipes 

• Sterile syringe (included in kit) 

•  Gloves (optional but recommended) 

•  Clamp (if not built into catheter tubing) 

Step-by-Step Instructions 

1.  Prepare 

   Wash your hands thoroughly with soap and water. 

   Put on gloves if available. 

   Clamp the catheter tubing about 3 inches below the sampling port. 

   Wait 15 minutes to allow urine to collect in the tubing. 

2. Clean the Sampling Port 
   Scrub the sampling port with an antiseptic wipe for at least 15 seconds. 
   Let it air dry completely. 

3. Collect the Sample 

   Remove the sterile syringe from its packaging. 
   Connect the syringe to the sampling port. 
   Slowly pull back the plunger to withdraw 10–20 mL of urine (3–5 mL is enough for culture; 10–20 mL for urinalysis). 
   Transfer the urine into the sterile container without touching the inside of the lid or container. 
   Test urine with urine test kit dip stick and await results. 

4. Secure and Store if urine is to be picked up by lab. 

• Close the container tightly.

• Wipe the outside if needed. 

• Place the container in the biohazard bag. 

• Unclamp the catheter tubing and ensure urine flows freely again. 

If sample is to be picked up and Transported to a laboratory 

• Best: Deliver to the lab or clinic within 1 hour. 

• If delayed: Store in the refrigerator (not freezer) and deliver within 24 hours. 

Avoid Before Collection 

• Drinking excessive water (can dilute bacteria) 

• Collecting during menstruation (if possible) 

• Using soaps, creams, or wipes near the area 

• Taking antibiotics, diuretics, or high-dose vitamin C unless advised otherwise 

Best Time to Collect 

• When symptoms are at their worst 

• First thing in the morning (urine is more concentrated) 
   
  Please call to schedule an appointment or speak with one of our triage nurses at 
  1-800-491-0909.  Select option 9 or simply say “triage.” 
  A nurse will answer or return your call promptly