Patient Education: Midstream Urine Collection for Home UTI Testing

Why This Matters 

Collecting a clean-catch midstream urine sample is important for accurate UTI testing. Improper collection can lead to contamination, false results, or unnecessary antibiotic use. 

What You’ll Need 

• Sterile specimen container (provided in your test kit) 

• Mild soap and water or cleansing wipes

• Clean towel or paper towels 

• Gloves (optional) 

• Biohazard transport bag 

Step-by-Step Instructions 

1.  Prepare 

   Wash your hands thoroughly with soap and water. 
   • If available, put on clean gloves. 
   • Open the sterile container without touching the inside of the lid or container. 

2. Clean the Area 

   • For Females: Separate the labia and clean from front to back using a
   cleansing wipe or soap and water. 
   • For Males: Retract the foreskin (if present) and clean the tip of the penis in a circular motion. 

3.  Collect the Sample 
   • Begin urinating into the toilet. 
   • After a few seconds, place the sterile container in the urine stream to collect the midstream portion. 
   • Remove the container before the urine stream ends. 
   • Finish urinating into the toilet.
   • Test urine using kit.  

4. If Specimen is to be collected Secure and Store 
   • Close the container tightly. 
   • Wipe the outside if needed. 
   • Place the container in the biohazard bag. 

Transporting the Sample 

• Best: Deliver to the lab or clinic within 1 hour. 
• If delayed: Store in the refrigerator (not freezer) and deliver within 24 hours. 
• If mailing: Follow the instructions provided with your test kit. 

Avoid Before Collection 

• Drinking excessive water (can dilute bacteria) 
• Collecting during menstruation (if possible) 
• Using soaps, creams, or wipes near the area just before collection 
• Taking antibiotics, diuretics, or high-dose vitamin C unless advised otherwise 

Best Time to Collect 

• When symptoms are at their worst 
• First thing in the morning (urine is more concentrated) 

Please call to schedule an appointment or speak with one of our triage nurses at 1-800-491-0909.  Select option 9 or simply say “triage.”  A nurse will answer or return your call promptly.